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(Plasmid #202591)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 202591 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone size w/o insert (bp) 6142
  • Total vector size (bp) 7647
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
  • Insert Size (bp)
  • Mutation
  • GenBank ID
    NM_001250856.1 KC295282.1
  • Entrez Gene
    APX1 (a.k.a. GLYMA_11G150243v4)
  • Tags / Fusion Proteins
    • mNeonGreen (N terminal on insert)
    • V5 epitope (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CCCTTGAACCTCCTCGTTCGACC
  • 3′ sequencing primer gggagaggggcgctagtcagtta
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mNeonGreen-APEX2 was a gift from Reuben Harris (Addgene plasmid # 202591 ; ; RRID:Addgene_202591)
  • For your References section:

    APEX3 - an optimized tool for rapid and unbiased proximity labeling. Becker JT, Auerbach AA, Harris RS. J Mol Biol. 2023 May 12:168145. doi: 10.1016/j.jmb.2023.168145. 10.1016/j.jmb.2023.168145 PubMed 37182813