gGBEv6.3-SpG
(Plasmid
#202630)
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Purposevector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpG nickase driven by EF1a promoter, guide RNA compatible with SpG driven by hU6, mCherry driven by CBh promoter
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Depositing Lab
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Depositing OrganizationHuidaGene Therapeutics Co., Ltd.
Located in China -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202630 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 202630-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 202630-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEF1a
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namenSpG(D10A)-MPGv6.3
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SpeciesSynthetic
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MutationSpG = D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R; MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202A, G203A, S206A, K210A, Q294R
- Promoter hU6, EF1a, CBh
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 202630-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 202630-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
gGBEv6.3-SpG was a gift from Huawei Tong (Addgene plasmid # 202630 ; http://n2t.net/addgene:202630 ; RRID:Addgene_202630) -
For your References section:
Programmable deaminase-free base editors for G-to-Y conversion by engineered glycosylase. Tong H, Liu N, Wei Y, Zhou Y, Li Y, Wu D, Jin M, Cui S, Li H, Li G, Zhou J, Yuan Y, Zhang H, Shi L, Yao X, Yang H. Natl Sci Rev. 2023 May 16;10(8):nwad143. doi: 10.1093/nsr/nwad143. eCollection 2023 Aug. 10.1093/nsr/nwad143 PubMed 37404457
