Salsa-BC1-P1A1
(Plasmid
#202842)
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PurposeExpression of barcoded reporter plasmid for PRESTO-Salsa; EGFP/BC1
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Depositing Lab
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Depositing OrganizationYale University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 202842 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 202842-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 202842-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCER206
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameEGFP/BC1
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SpeciesSynthetic
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Insert Size (bp)730
- Promoter Tet Response Element (TRE)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsrGI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer Custom primer (GAAGCGCGATCACATGG)
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 202842-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 202842-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Salsa-BC1-P1A1 was a gift from Noah Palm (Addgene plasmid # 202842 ; http://n2t.net/addgene:202842 ; RRID:Addgene_202842) -
For your References section:
Highly multiplexed bioactivity screening reveals human and microbiota metabolome-GPCRome interactions. Chen H, Rosen CE, Gonzalez-Hernandez JA, Song D, Potempa J, Ring AM, Palm NW. Cell. 2023 Jul 6;186(14):3095-3110.e19. doi: 10.1016/j.cell.2023.05.024. Epub 2023 Jun 14. 10.1016/j.cell.2023.05.024 PubMed 37321219
