|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20318||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5133
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Hygromycin, 200 μg/mL
Copy numberLow Copy
Speciessynthetic gene derived from Tn10 tetR
Insert Size (bp)970
- Cloning method Restriction Enzyme
- 5′ cloning site PacI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
There are minor discrepancies between depositor's and Addgene's sequence. Depositor is aware of these and thinks they should not have any affect on function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTE-10M0X was a gift from Dirk Schnappinger (Addgene plasmid # 20318 ; http://n2t.net/addgene:20318 ; RRID:Addgene_20318)
For your References section:Improved tetracycline repressors for gene silencing in mycobacteria. Klotzsche M, Ehrt S, Schnappinger D. Nucleic Acids Res. 2009 Jan 27. ():. 10.1093/nar/gkp015 PubMed 19174563