pV132-vCas9
(Plasmid #203223)

• Purpose: Mammalian expression of vCas9
• Depositing Labs: Vikash Chauhan, Robert Langer, Phil Sharp
• Publication: Chauhan et al Proc Natl Acad Sci U S A. 2023 Mar 14;120(11):e2300605120. doi: 10.1073/pnas.2300605120. Epub 2023 Mar 7.

Backbone

• Vector backbone: pX165
• Total vector size (bp): 8214
• Vector type: Mammalian Expression, AAV, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: vCas9
• Species: Synthetic; S. pyogenes
• Insert Size (bp): 4272
• Mutation: S55R R976A K1003A T1314R
• Promoter: CBh

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BshTI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: CBh-FWD
• 3′ sequencing primer: bGH-REV

Resource Information

• Supplemental Documents:
  • pV132-vCas9.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry