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(Plasmid #20336)


Item Catalog # Description Quantity Price (USD)
Plasmid 20336 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
    pRset B
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2764
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Perceval (fluorescent ATP:ADP sensor)
  • Species
  • Insert Size (bp)
  • Tag / Fusion Protein
    • his(7) (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 reverse primer
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    synthetic gene
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Articles Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRsetB-his7-Perceval was a gift from Gary Yellen (Addgene plasmid # 20336 ; ; RRID:Addgene_20336)
  • For your References section:

    A genetically encoded fluorescent reporter of ATP:ADP ratio. Berg J, Hung YP, Yellen G. Nat Methods. 2009 Feb . 6(2):161-6. 10.1038/nmeth.1288 PubMed 19122669