pCAG-FIP200 (1-640)-TSF
(Plasmid #203545)

Purpose

FIP200 NTD-TwinStrep-Flag mammalian expression
Depositing Lab

James Hurley
Publication

Hurley lab mammalian expression plasmids (unpublished) ( How to cite )
Sequence Information
- Sequences (3)

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Plasmid	203545	Standard format: Plasmid sent in bacteria as agar stab	1	$85	Add to Cart

This material is available to academics and nonprofits only.

Backbone

Vector backbone
pCAG
Vector type
Mammalian Expression

Growth in Bacteria

Bacterial Resistance(s)
Ampicillin, 100 μg/mL
Growth Temperature
37°C
Growth Strain(s)
DH5alpha
Copy number
High Copy

Gene/Insert

Gene/Insert name
FIP200
Species
H. sapiens (human)
GenBank ID
NP_055596.3
Entrez Gene
RB1CC1 (a.k.a. ATG17, CC1, FIP200, PPP1R131)
Promoter CMV
Tag / Fusion Protein
- TwinStrep Flag (C terminal on insert)

Cloning Information

Cloning method Restriction Enzyme
5′ cloning site ClaI (unknown if destroyed)
3′ cloning site kpnI (unknown if destroyed)

Terms and Licenses

Academic/Nonprofit Terms
- UBMTA
Industry Terms
- Not Available to Industry

Trademarks:

Zeocin® is an InvivoGen trademark.

Depositor Comments

FIP200 (1-640)-TEVsite-TwinStrep-Flag-stop

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

For your Materials & Methods section:

pCAG-FIP200 (1-640)-TSF was a gift from James Hurley (Addgene plasmid # 203545 ; http://n2t.net/addgene:203545 ; RRID:Addgene_203545)

pCAG-FIP200 (1-640)-TSF (Plasmid #203545)