Structure and activation of the human autophagy-initiating ULK1C:PI3KC3-C1 supercomplex
Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH
bioRxiv 2023.06.01.543278 Article
Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH
bioRxiv 2023.06.01.543278 Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 203542 | pCAG-FIP200 (1-640) | Make FIP200 NTD:ATG13:ULK1 complex |
| 203543 | pCAG-FIP200(1-640)-MBP | FIP200 NTD-GFP mammalian expression |
| 203544 | pCAG-FIP200 (1-640)-GST | FIP200 NTD-MBP mammalian expression |
| 203545 | pCAG-FIP200 (1-640)-TSF | FIP200 NTD-TwinStrep-Flag mammalian expression |
| 203546 | pCAG-FIP200 (1-640)-msfGFP | FIP200 NTD-GFP-10His mammalian expression |
| 203547 | pCAG-TSF-ATG13 (363-517)-ULK1 (836-1050) | Mammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complex |
| 203548 | pCAG-TSF-ATG13 (450-517)-ULK1 (836-1050) | Mammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complex |
| 203549 | pCAG-ATG13 (363-517)-ULK1 (836-1050)-TSF | Mammalian expression of ATG13 C-terminal and ULK1 MIT domain fusion complex |
| 203550 | pCAG-TSF-ATG13 (363-517) | Mammalian expression of TwinStrep-Flag tagged ATG13 (363-517) |
| 203551 | pCAG-GST-ATG13 (363-517) | Mammalian expression of GST tagged ATG13 (363-517) |
| 203552 | pCAG-GST-ATG13 (460-517) | Mammalian expression of GST tagged ATG13 (460-517) |
| 203553 | pCAG-GST-ATG13 (363-460) | Mammalian expression of GST tagged ATG13 (363-460) |
| 203554 | pCAG-GST-ATG13 (363-398) | Mammalian expression of GST tagged ATG13 (363-398) |
| 203555 | pCAG-GST-ATG13 (363-392) | Mammalian expression of GST tagged ATG13 (363-392) |
| 203556 | pCAG-GST-ATG13 (402-517) | Mammalian expression of GST tagged ATG13 (402-517) |
| 203557 | pCAG-GST-ATG13 (402-450) | Mammalian expression of GST tagged ATG13 (402-450) |
| 203558 | pCAG-GST-ATG13 (450-517) | Mammalian expression of GST tagged ATG13 (450-517) |
| 203559 | pCAG-GST-ATG13 (6-196) | Mammalian expression of GST tagged ATG13 (6-196) |
| 203560 | pCAG-msfGFP-ATG13 (1-197) | Mammalian expression of GFP tagged ATG13 (1-197) |
| 203561 | pCAG-ATG13 (363-517)-MBP | Mammalian expression of ATG13 (363-517) with MBP tag |
| 203562 | pCAG-ULK1 (836-1050)-MBP | Mammalian expression of ULK1 (836-1050) with MBP tag |
| 203563 | pCAG-VPS15-TSF | Mammalian expression of VPS15 with TwinStrep-Flag tag |
| 203564 | pCAG-VPS34 | Mammalian expression of VPS34 |
| 203565 | pCAG-ATG14 | Mammalian expression of ATG14 |
| 203566 | pCAG-GST-ATG14 | Mammalian expression of GST tagged ATG14 |
| 203567 | pCAG-mCherry-ATG14 | Mammalian expression of mCherry tagged ATG14 |
| 203568 | pCAG-BECN1 | Mammalian expression of BECN1 |
| 210839 | pCAG-FIP200 (1-640)-V314D-TSF | Mammalian expression of FIP200 NTD mutant |
| 210840 | pCAG-FIP200 (1-640)-R326D-TSF | Mammalian expression of FIP200 NTD mutant |
| 210841 | pCAG-FIP200 (1-640)-R334D-TSF | Mammalian expression of FIP200 NTD mutant |
| 210842 | pCAG-FIP200 (1-640)-L324D/R543D/R548D-TSF | Mammalian expression of FIP200 NTD mutant |
| 210843 | pCAG-FIP200 (1-640)-V44D-TSF | Mammalian expression of FIP200 NTD mutant |
| 210844 | pCAG-GST-ATG13 (460-517)-F466D/F470D | Mammalian expression of ATG13 (460-517) mutant |
| 210845 | pCAG-GST-ATG13 (460-517)-L476/L479D | Mammalian expression of ATG13 (460-517) mutant |
| 210846 | pCAG-GST-ATG13 (460-517)-L495/L499D | Mammalian expression of ATG13 (460-517) mutant |
| 210847 | pCAG-GST-ATG13 (460-517)-F509/F512D | Mammalian expression of ATG13 (460-517) mutant |
| 210848 | pCAG-GST-ATG13 (363-398)-F394D/M396D/F397D | Mammalian expression of ATG13 (363-398) mutant |
| 210849 | pCAG-GST-ATG13 (363-450) | Mammalian expression of ATG13 (363-450) mutant |
| 210850 | pCAG-GST-ATG13 (363-450)-F394D/F397D /E403K | Mammalian expression of ATG13 (363-450) mutant |
| 210851 | pCAG-GST-ATG13 (450-517)-A452D/F453D | Mammalian expression of ATG13 (450-517) mutant |
| 210852 | pCDH1-CMV-FIP200-mCherry-Hygro | Lentiviral expression vector encoding FIP200-mCherry |
| 210853 | pCDH1-CMV-FIP200-V44D-mCherry-Hygro | Lentiviral expression vector encoding FIP200 mutant-mCherry |
| 210854 | pCDH1-CMV-FIP200-V314D-mCherry-Hygro | Lentiviral expression vector encoding FIP200 mutant-mCherry |
| 210855 | pCDH1-CMV-FIP200-V44D/V314D-mCherry-Hygro | Lentiviral expression vector encoding FIP200 mutant-mCherry |
| 210856 | pCDH1-CMV-FIP200-L324D/R543D/R548D-mCherry-Hygro | Lentiviral expression vector encoding FIP200 mutant-mCherry |
| 210857 | pCDH1-CMV-FIP200-V44D/V314D/L324D/R543D/R548D-mCherry-Hygro | Lentiviral expression vector encoding FIP200 mutant-mCherry |
| 210858 | pCAG-TSF-ATG101 | Mammalian expression of ATG101 |