Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pCAG-ATG13 (363-517)-ULK1 (836-1050)-TSF
(Plasmid #203549)


Item Catalog # Description Quantity Price (USD)
Plasmid 203549 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    ATG13, ULK1
  • Species
    H. sapiens (human)
  • GenBank ID
    NP_001333259.1, NP_003556.2
  • Entrez Gene
    ATG13 (a.k.a. KIAA0652, PARATARG8)
  • Entrez Gene
    ULK1 (a.k.a. ATG1, ATG1A, UNC51, Unc51.1, hATG1)
  • Promoter CMV
  • Tag / Fusion Protein
    • TwinStrep Flag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

ATG13 (450-517)-5aa linker-ULK1 (836-1050)-TEVsite-TwinStrep-Flag-stop

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-ATG13 (363-517)-ULK1 (836-1050)-TSF was a gift from James Hurley (Addgene plasmid # 203549 ; ; RRID:Addgene_203549)
  • For your References section:

    Structure and activation of the human autophagy-initiating ULK1C:PI3KC3-C1 supercomplex. Chen M, Ren X, Nguyen TN, Khuu G, Cook ASI, Lazarou M, Hurley JH. bioRxiv 2023.06.01.543278 10.1101/2023.06.01.543278