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pCAG-FIP200 (1-640)-msfGFP
(Plasmid #203546)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203546 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 203546-D50 50 μg of DNA in Tris buffer $495
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DNA 203546-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pCAG
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    FIP200
  • Species
    H. sapiens (human)
  • GenBank ID
    NP_055596.3
  • Entrez Gene
    RB1CC1 (a.k.a. ATG17, CC1, FIP200, PPP1R131)
  • Promoter CMV
  • Tag / Fusion Protein
    • msfGFP-10His (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site ClaI (unknown if destroyed)
  • 3′ cloning site KpnI (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

FIP200 (1-640)-TEVsite-msfGFP-10His-stop

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCAG-FIP200 (1-640)-msfGFP was a gift from James Hurley (Addgene plasmid # 203546 ; http://n2t.net/addgene:203546 ; RRID:Addgene_203546)

  • For your References section:

    Structure and activation of the human autophagy-initiating ULK1C:PI3KC3-C1 supercomplex. Chen M, Nguyen TN, Ren X, Khuu G, Cook ASI, Zhao Y, Yildiz A, Lazarou M, Hurley JH. Nat Struct Mol Biol. 2025 Sep;32(9):1596-1605. doi: 10.1038/s41594-025-01557-x. Epub 2025 May 29. 10.1038/s41594-025-01557-x PubMed 40442316
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