pCDNA3 Flag p38 beta2 (agf)
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20356||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5500
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
Gene/Insert namepDCNA3 Flag p38 beta(agf)
SpeciesH. sapiens (human)
Insert Size (bp)1212
MutationReplace Thr 182 and Tyr 184 with Ala and Phe respectively
Entrez GeneMAPK11 (a.k.a. P38B, P38BETA2, PRKM11, SAPK2, SAPK2B, p38-2, p38Beta)
/ Fusion Protein
- Flag (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Hind III (not destroyed)
- 3′ cloning site Xho 1 (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
There are two additional mutations in the sequence - Q22L and M198L. These mutations are correct and the sequence still functions normally.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCDNA3 Flag p38 beta2 (agf) was a gift from Roger Davis (Addgene plasmid # 20356 ; http://n2t.net/addgene:20356 ; RRID:Addgene_20356)
For your References section:Selective activation of p38 mitogen-activated protein (MAP) kinase isoforms by the MAP kinase kinases MKK3 and MKK6. Enslen H, Raingeaud J, Davis RJ. J Biol Chem. 1998 Jan 16. 273(3):1741-8. 10.1074/jbc.273.3.1741 PubMed 9430721