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pOSML-Cox2-GFP
(Plasmid #203711)

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Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 203711 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 203711-D50 50 μg of DNA in Tris buffer $495
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DNA 203711-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pOSML
  • Backbone size w/o insert (bp) 5000
  • Modifications to backbone
    pOSML was modified from pMT2 by adding a larger polylinker sequence (PMID:7965814). The GFP tag was inserted at BspEI sist in Cox2 coding sequence. GFP is inserted 18 aa upstream of the C-terminal end of the protein (PMID:10567385).
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cox2-GFP
  • Alt name
    PTGS2-GFP
  • Species
    H. sapiens (human)
  • Mutation
    WT
  • Entrez Gene
    PTGS2 (a.k.a. COX-2, COX2, GRIPGHS, PGG/HS, PGHS-2, PHS-2, hCox-2)
  • Promoter SV40
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal1 (unknown if destroyed)
  • 3′ cloning site Sal1 (unknown if destroyed)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOSML-Cox2-GFP was a gift from Timothy Hla (Addgene plasmid # 203711 ; http://n2t.net/addgene:203711 ; RRID:Addgene_203711)

  • For your References section:

    Overexpression of cyclooxygenase-2 induces cell cycle arrest. Evidence for a prostaglandin-independent mechanism. Trifan OC, Smith RM, Thompson BD, Hla T. J Biol Chem. 1999 Nov 26;274(48):34141-7. PubMed 10567385
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