Clomeleon
(Plasmid
#203723)
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PurposeA genetically encoded ratiometric optical indicator for chloride ions, to detect changes in intracellular chloride concentration
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Depositing Lab
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Depositing OrganizationDuke University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203723 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 203723-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 203723-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepRK5
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Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namecyan fluorescent protein linked yellow florescent protein
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SpeciesSynthetic
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MutationCyan Fluorescent Protein, Topaz Fluorescent Protein, rTEV protease cleavage site, His6 tag
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 203723-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 203723-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Clomeleon was a gift from George Augustine (Addgene plasmid # 203723 ; http://n2t.net/addgene:203723 ; RRID:Addgene_203723) -
For your References section:
A genetically encoded ratiometric indicator for chloride: capturing chloride transients in cultured hippocampal neurons. Kuner T, Augustine GJ. Neuron. 2000 Sep;27(3):447-59. doi: 10.1016/s0896-6273(00)00056-8. 10.1016/s0896-6273(00)00056-8 PubMed 11055428
Map uploaded by the depositor.
