SuperClomeleon
(Plasmid #203724)

• Purpose: An improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride
• Depositing Lab: George Augustine
• Publication: Grimley et al J Neurosci. 2013 Oct 9;33(41):16297-309. doi: 10.1523/JNEUROSCI.4616-11.2013.

Backbone

• Vector backbone: pRK5
• Vector type: Mammalian Expression, Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Cerulean linked topaz fluorescent protein
• Species: Synthetic
• Mutation: Cerulean, modified Topaz Fluorescent Protein with enhanced halide binding affinity, C-terminal Flag, His6 epitope tags

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: unknown

Resource Information

• Supplemental Documents:
  • 203724: SuperClomeleon

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry