SuperClomeleon
(Plasmid
#203724)
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PurposeAn improved engineered fluorescent ratiometric optogenetic sensor to detect inhibitory synaptic activity via changes in intracellular chloride
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Depositing Lab
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Depositing OrganizationNanyang Technological University
Located in Singapore -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 203724 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 203724-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 203724-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepRK5
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Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameCerulean linked topaz fluorescent protein
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SpeciesSynthetic
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MutationCerulean, modified Topaz Fluorescent Protein with enhanced halide binding affinity, C-terminal Flag, His6 epitope tags
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer unknown
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 203724-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 203724-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
SuperClomeleon was a gift from George Augustine (Addgene plasmid # 203724 ; http://n2t.net/addgene:203724 ; RRID:Addgene_203724) -
For your References section:
Visualization of synaptic inhibition with an optogenetic sensor developed by cell-free protein engineering automation. Grimley JS, Li L, Wang W, Wen L, Beese LS, Hellinga HW, Augustine GJ. J Neurosci. 2013 Oct 9;33(41):16297-309. doi: 10.1523/JNEUROSCI.4616-11.2013. 10.1523/JNEUROSCI.4616-11.2013 PubMed 24107961
Map uploaded by the depositor.
