pGP-CMV-jGCaMP8f F366H
(Plasmid #204141)

• Purpose: Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutation
• Depositing Lab: Katalin Torok
• Depositing Organization: City, University of London
  Located in the United Kingdom of Great Britain and Northern Ireland
• Publication: Tran et al Front Cell Neurosci. 2023 May 18;17:1155406. doi: 10.3389/fncel.2023.1155406. eCollection 2023.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: ThermoFisher Invitrogen
• Total vector size (bp): 5224
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: jGCaMP8f F366H
• Species: R. norvegicus (rat), G. gallus (chicken), Synthetic; A. victoria (jellyfish)
• Insert Size (bp): 1269
• Mutation: Phenylalanine 366 changed to histidine
• Promoter: CMV
• Tag / Fusion Protein:
  • N-terminal His tag (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40 pA-R

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Addgene plasmid 162373 (deposited by GENIE Project)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pGP-CMV-jGCaMP8f was a gift from GENIE Project (Addgene plasmid # 162373 ; http://n2t.net/addgene:162373 ; RRID:Addgene_162373)

We introduced the F366H mutation by site-directed mutagenesis

DNA (Catalog # 204141-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 204141-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.