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CMV5.1-SARS-CoV-2 Nsp3
(Plasmid #204205)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 204205 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 204205-D50 50 μg of DNA in Tris buffer $495
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DNA 204205-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    CMV5.1p
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV-2 Nsp3 (Wuhan-Hu-1; https://www.addgene.org/141257/)
  • Species
    Other
  • Mutation
    none, from obtained clone
  • Entrez Gene
    ORF1ab (a.k.a. GU280_gp01)
  • Tag / Fusion Protein
    • FLAGx3 (N terminal on insert)

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' Cloning Site: ClaI (not destroyed), 3' Cloning Site: NotI (not destroyed). Contains entire SARS-CoV-2 Nsp3 from Wuhan isolate.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV5.1-SARS-CoV-2 Nsp3 was a gift from Allan Weissman (Addgene plasmid # 204205 ; http://n2t.net/addgene:204205 ; RRID:Addgene_204205)

  • For your References section:

    SARS-CoV-2 papain-like protease plays multiple roles in regulating cellular proteins in the endoplasmic reticulum. Yang M, Mariano J, Su R, Smith CE, Das S, Gill C, Andresson T, Loncarek J, Tsai YC, Weissman AM. J Biol Chem. 2023 Oct 12;299(12):105346. doi: 10.1016/j.jbc.2023.105346. 10.1016/j.jbc.2023.105346 PubMed 37838170
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