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SARS-CoV-2 papain-like protease plays multiple roles in regulating cellular proteins in the endoplasmic reticulum.

Yang M, Mariano J, Su R, Smith CE, Das S, Gill C, Andresson T, Loncarek J, Tsai YC, Weissman AM
J Biol Chem. 2023 Oct 12;299(12):105346. doi: 10.1016/j.jbc.2023.105346. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
204192pCDNA3- SREBP-1Mammalian expression of SREBP-1 (isoform 1)
204193pCDNA3-SREBP-1 (A317-318)Mammalian expression of SREBP-1 (isoform 1)
204194pCDNA3-SREBP-1 (A317-318; A341)Mammalian expression of SREBP-1 (Isoform 1)
204195pCDNA3- SREBP-1 (A317-318; L340)Mammalian expression of SREBP-1 (Isoform 1)
204196pCDNA3- SREBP-1 (A317-318;A341; A1171)Mammalian expression of SREBP-1 (isoform 1)
204197pCDNA3-SREBP-1 (A341; A1171)Mammalian expression of SREBP-1 (isoform 1)
204198pCDNA3-SREBP-1-GFP (A317-318; A341)Mammalian expression of SREBP-1 (isoform 1)
204199pCDNA3-SREBP-1-GFP (A317-318; A341; A1171)Mammalian expression of SREBP-1 (isoform 1)
204200pCDNA3-SREBP-2 Mammalian expression of SREBP-2
204201pCDNA3-SREBP-2 (A1134-1135)Mammalian expression of SREBP-2
204202pCDNA3-SREBP-2-GFPMammalian expression of SREBP-2
204203pCDNA3-SREBP-2-GFP (A1134-1135)Mammalian expression of SREBP-2
204204pCDNA3-SREBP-2 (STOP)Mammalian expression of SREBP-2
204205CMV5.1-SARS-CoV-2 Nsp3Mammalian expression of SARS-CoV-2 Nsp3
204206pCMV-SARS-CoV-2 Nsp3TMMammalian expression of SARS-CoV-2 Nsp3
204207pCMV-SARS-CoV-2 Nsp3TMC111AMammalian expression of SARS-CoV-2 Nsp3
204208pCMV-SARS-CoV-2-Nsp3PLproMammalian expression of SARS-CoV-2 Nsp3
210767pCDNA3-SREBP-1 S1P and S2P mutations (U124AFG200-5)Mammalian expression of SREBP-1 (isoform 1) with mutations in residues reported to be critical for S1P and S2P cleavage.

Antibodies from Article