CMV-Halo-10-PFN1-G118V
(Plasmid #204225)

• Purpose: Expression of Halo-Profilin-1(G118V) in tissue culture cells
• Depositing Lab: Jessica Henty-Ridilla
• Publication: Pimm et al Elife. 2022 Jun 6;11:e76485. doi: 10.7554/eLife.76485.

Backbone

• Vector backbone: pcDNA-Halo expression vector
• Backbone manufacturer: Gunther Hollopeter, Cornell University
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PFN1
• Species: H. sapiens (human)
• Mutation: G118V
• Entrez Gene: PFN1 (a.k.a. ALS18)
• Promoter: CMV
• Tag / Fusion Protein:
  • Halo, TEV site, SGLRSRAQAS linker (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: NotI (unknown if destroyed)

Resource Information

• Supplemental Documents:
  • CMV-Halo-10-PFN1-G118V.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

PFN1(G118V) was synthesized and inserted into Halo-expression vector by Genscript