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(Plasmid #186272)


Item Catalog # Description Quantity Price (USD)
Plasmid 186272 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 5368
  • Total vector size (bp) 5978
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    GTP-binding nuclear protein Ran
  • Alt name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    Changed Q69 into L
  • GenBank ID
  • Promoter T7 promoter
  • Tag / Fusion Protein
    • His-tagged at N terminal (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer TAATA CGACT CACTA TAGGG
  • 3′ sequencing primer ATGCT AGTTA TTGCT CAGCG G
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

For protein expression, transform into BL21(DE3) E. coli because the pET vector system requires T7 RNA polymerase for expression. Induce with 1 mM ITPG for 2h at 28 °C.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28b_Q69L was a gift from Masahito Ikawa (Addgene plasmid # 186272 ; ; RRID:Addgene_186272)
  • For your References section:

    RanGTP and the actin cytoskeleton keep paternal and maternal chromosomes apart during fertilization. Mori M, Yao T, Mishina T, Endoh H, Tanaka M, Yonezawa N, Shimamoto Y, Yonemura S, Yamagata K, Kitajima TS, Ikawa M. J Cell Biol. 2021 Oct 4;220(10). pii: 212591. doi: 10.1083/jcb.202012001. Epub 2021 Aug 23. 10.1083/jcb.202012001 PubMed 34424312