hKCNQ3(WT).ires.mCherry-pcDNA3
(Plasmid
#204362)
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PurposeHeterologous expression in mammalian cell lines or Xenopus oocyte (T7 RNA pol)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 204362 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKCNQ3
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SpeciesH. sapiens (human)
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GenBank IDNM_004519.4
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Entrez GeneKCNQ3 (a.k.a. BFNC2, EBN2, KV7.3)
- Promoter CMV/T7 RNApol
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
KCNQ3-202 splice variant (Ensembl).
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
hKCNQ3(WT).ires.mCherry-pcDNA3 was a gift from Aguan Wei (Addgene plasmid # 204362 ; http://n2t.net/addgene:204362 ; RRID:Addgene_204362) -
For your References section:
Human KCNQ5 de novo mutations underlie epilepsy and intellectual disability. Wei AD, Wakenight P, Zwingman TA, Bard AM, Sahai N, Willemsen MH, Schelhaas HJ, Stegmann APA, Verhoeven JS, de Man SA, Wessels MW, Kleefstra T, Shinde DN, Helbig KL, Basinger A, Wagner VF, Rodriguez-Buritica D, Bryant E, Millichap JJ, Millen KJ, Dobyns WB, Ramirez JM, Kalume FK. J Neurophysiol. 2022 Jul 1;128(1):40-61. doi: 10.1152/jn.00509.2021. Epub 2022 May 18. 10.1152/jn.00509.2021 PubMed 35583973
