pDisplay-SNAPtag
(Plasmid #205094)

• Purpose: Display SNAPtag on the surface of mammalian cells
• Depositing Lab: Bianxiao Cui
• Publication: Zhang et al Nat Cell Biol. 2023 Sep 28. doi: 10.1038/s41556-023-01238-1.

Backbone

• Vector backbone: pCMV(MinDis) [variant of pDisplay]
• Backbone size w/o insert (bp): 5300
• Total vector size (bp): 5832
• Modifications to backbone: From pDisplay: Hemagglutinin (HA) tag removed
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SNAPtag
• Insert Size (bp): 546

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BglII (not destroyed)
• 3′ cloning site: SalI (not destroyed)
• 5′ sequencing primer: GTG ACA GAT CTG CCG CAG GCA GCA TGG ACA AAG ACT GCG AAA TGA AGC
• 3′ sequencing primer: TGT TCG TCG ACC TGC AGC GAG CTG CCC AGC CCA G

Resource Information

• Supplemental Documents:
  • pDisplay SNAPtag.gb
• A portion of this plasmid was derived from a plasmid made by: The plasmid backbone was amplified from pCMV(MinDis).iGluSnFR, a gift from Loren Looger (Addgene plasmid # 41732).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry