pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2
(Plasmid #205161)

• Purpose: express β-ACTIN-3′UTR-24xMS2
• Depositing Lab: Weirui Ma
• Publication: Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.7554/eLife.82178.

Backbone

• Vector backbone: pcDNA
• Total vector size (bp): 8742
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: β-ACTIN-3′UTR-24xMS2
• Insert Size (bp): 2929
• Promoter: CMV
• Tag / Fusion Protein:
  • tagBFP (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CCACATATAGATCCAAGAAACCCGC
• 3′ sequencing primer: TAGAAGGCACAGTCGAGG

Resource Information

• Supplemental Documents:
  • pcDNA-puro-tagBFP-β-ACTIN-3′UTR-24xMS2.gbk
• A portion of this plasmid was derived from a plasmid made by: the β-ACTIN coding sequence with a 3′UTR of 373 bp was PCR amplified from the cDNA of HEK293T/17 cells and the 24xMS2 fragment was amplified from plasmid "pcDNA-puro-GFP-24xMS2"

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry