pHR- flag-Lag16-MUC4
(Plasmid
#205198)
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PurposeThis vector encodes of the synCAM (MUC4) and GFP nanobody LAG16
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205198 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepHR
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Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namesynCAM MUC4 and Lag16 anti-GFP nanobody
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SpeciesSynthetic
Resource Information
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A portion of this plasmid was derived from a plasmid made byThe GFP nanobodies are from the Rout lab. Please see: A robust pipeline for rapid production of versatile nanobody repertoires. Nature Methods. 2014;11:1253–1260.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHR- flag-Lag16-MUC4 was a gift from Wendell Lim (Addgene plasmid # 205198 ; http://n2t.net/addgene:205198 ; RRID:Addgene_205198) -
For your References section:
Programming multicellular assembly with synthetic cell adhesion molecules. Stevens AJ, Harris AR, Gerdts J, Kim KH, Trentesaux C, Ramirez JT, McKeithan WL, Fattahi F, Klein OD, Fletcher DA, Lim WA. Nature. 2023 Feb;614(7946):144-152. doi: 10.1038/s41586-022-05622-z. Epub 2022 Dec 12. 10.1038/s41586-022-05622-z PubMed 36509107
