pTol2-elavl3-WHaloCaMP1a
(Plasmid
#205312)
-
PurposeExpression of WHaloCaMP1a in zebrafish from the elavl3 promoter (pan-neuronal expression)
-
Depositing Lab
-
Depositing OrganizationHHMI, Janelia Research Campus
Located in United States of America -
Citations
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205312 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonepTol2
-
Vector typeZebrafish expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameWHaloCaMP1a
-
SpeciesSynthetic
Cloning Information
- Cloning method Gibson Cloning
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.07.18.549527 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pTol2-elavl3-WHaloCaMP1a was a gift from Eric Schreiter (Addgene plasmid # 205312 ; http://n2t.net/addgene:205312 ; RRID:Addgene_205312) -
For your References section:
A modular chemigenetic calcium indicator for multiplexed in vivo functional imaging. Farrants H, Shuai Y, Lemon WC, Monroy Hernandez C, Zhang D, Yang S, Patel R, Qiao G, Frei MS, Plutkis SE, Grimm JB, Hanson TL, Tomaska F, Turner GC, Stringer C, Keller PJ, Beyene AG, Chen Y, Liang Y, Lavis LD, Schreiter ER. Nat Methods. 2024 Oct;21(10):1916-1925. doi: 10.1038/s41592-024-02411-6. Epub 2024 Sep 20. 10.1038/s41592-024-02411-6 PubMed 39304767