CMV HA-MADD
(Plasmid
#206150)
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PurposeCMV HA-MADD
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 206150 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRK5-HA vector
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Backbone manufacturerAdapted from Addgene# 17613
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 10200
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Modifications to backbonereplaced Parkin with MADD
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMADD
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SpeciesH. sapiens (human)
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Insert Size (bp)5500
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Entrez GeneMADD (a.k.a. DEEAH, DENN, IG20, NEDDISH, RAB3GEP, RabGEF)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (filled-in) / HindIII (filled-in); HindIII restored (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer 5’- ATT TAG GTG ACA CTA TAG AA (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMADD cDNA from Transomic Technologies
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV HA-MADD was a gift from Erika Holzbaur (Addgene plasmid # 206150)