CMV HA-MADD
(Plasmid
#206150)
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PurposeCMV HA-MADD
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Depositing Lab
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Depositing OrganizationUniversity of Pennsylvania
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 206150 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 206150-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 206150-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepRK5-HA vector
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Backbone manufacturerAdapted from Addgene# 17613
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 10200
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Modifications to backbonereplaced Parkin with MADD
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMADD
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SpeciesH. sapiens (human)
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Insert Size (bp)5500
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Entrez GeneMADD (a.k.a. DEEAH, DENN, IG20, NEDDISH, RAB3GEP, RabGEF)
- Promoter CMV
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Tag
/ Fusion Protein
- HA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SalI (filled-in) / HindIII (filled-in); HindIII restored (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer 5’- ATT TAG GTG ACA CTA TAG AA
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMADD cDNA from Transomic Technologies
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 206150-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 206150-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CMV HA-MADD was a gift from Erika Holzbaur (Addgene plasmid # 206150 ; http://n2t.net/addgene:206150 ; RRID:Addgene_206150) -
For your References section:
RAB3 phosphorylation by pathogenic LRRK2 impairs trafficking of synaptic vesicle precursors. Dou D, Aiken J, Holzbaur ELF. J Cell Biol. 2024 Jun 3;223(6):e202307092. doi: 10.1083/jcb.202307092. Epub 2024 Mar 21. 10.1083/jcb.202307092 PubMed 38512027
