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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20693 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEX-6p-1
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Backbone manufacturerGE
- Backbone size w/o insert (bp) 6300
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsDH5a, 37 degrees
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameProgrammed Cell Death Protein 4
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Alt namePdcd4
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SpeciesH. sapiens (human)
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Insert Size (bp)1410
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MutationI36V- does not alter function
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GenBank IDNM_014456
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Entrez GenePDCD4 (a.k.a. H731)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX6p1-hPdcd4 was a gift from Haiwei Song (Addgene plasmid # 20693 ; http://n2t.net/addgene:20693 ; RRID:Addgene_20693) -
For your References section:
Structural basis for translational inhibition by the tumour suppressor Pdcd4. Loh PG, Yang HS, Walsh MA, Wang Q, Wang X, Cheng Z, Liu D, Song H. EMBO J. 2009 Feb 4. 28(3):274-85. 10.1038/emboj.2008.278 PubMed 19153607