pCAS
(Plasmid #206994)

• Purpose: Plasmid expressing both SpCas9 and SaCas9
• Depositing Lab: Alejandro Chavez
• Depositing Organization: University of California, San Diego (UCSD)
  Located in United States of America
• Publication: Kim et al Sci Adv. 2024 May 3;10(18):eadg8771. doi: 10.1126/sciadv.adg8771. Epub 2024 May 1.

Backbone

• Vector backbone: pCSD_2xNLS-SpCas9WT-NLS-3xHA-NLS-SaCas9WT-2xNLS
• Modifications to backbone: 3xHA to P2A
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SpCas9 - P2A - SaCas9
• Alt name: Staphylococcus aureus Cas9
• Alt name: Streptococcus pyogenes Cas9
• Entrez Gene: NEWENTRY
• Entrez Gene: NEWENTRY
• Promoter: CMV
• Tag / Fusion Protein:
  • P2A

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: P2A binding primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/10.1101/2022.11.16.516691v1 for bioRxiv preprint.

DNA (Catalog # 206994-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 206994-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.