Addgene: High-throughput tagging of endogenous loci for rapid characterization of protein function.

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High-throughput tagging of endogenous loci for rapid characterization of protein function.
Kim J, Kratz AF, Chen S, Sheng J, Kim HK, Zhang L, Singh BK, Chavez A
Sci Adv. 2024 May 3;10(18):eadg8771. doi: 10.1126/sciadv.adg8771. Epub 2024 May 1. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
206990	pDNR-gRNA	A plasmid for expression of SaCas9-sgRNA targeting donor plasmids
206991	pDNR-mCherry-T2A-3xPuroR-Frame 0	Donor plasmid for Frame 0 with mCherry tag and triple Puromycin resistant marker (3xPuroR)
206992	pDNR-mCherry-T2A-3xPuroR-Frame 1	Donor plasmid for Frame 1 with mCherry tag and triple Puromycin resistant marker (3xPuroR)
206993	pDNR-mCherry-T2A-3xPuroR-Frame 2	Donor plasmid for Frame 2 with mCherry tag and triple Puromycin resistant marker (3xPuroR)
206994	pCAS	Plasmid expressing both SpCas9 and SaCas9

Plasmids from Article

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