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pcDNA3-myc3-RBBP4
(Plasmid #20715)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 20715 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 4620
  • Vector type
    Mammalian Expression
  • Selectable markers
    G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    retinoblastoma binding protein 4
  • Alt name
    RBBP4
  • Species
    H. sapiens (human)
  • Entrez Gene
    RBBP4 (a.k.a. NURF55, RBAP48, lin-53)
  • Tag / Fusion Protein
    • myc3 (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Data not available (not destroyed)
  • 3′ cloning site Data not available (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SP6
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
  • Article Citing this Plasmid
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-myc3-RBBP4 was a gift from Yue Xiong (Addgene plasmid # 20715 ; http://n2t.net/addgene:20715 ; RRID:Addgene_20715)
  • For your References section:

    DDB1 functions as a linker to recruit receptor WD40 proteins to CUL4-ROC1 ubiquitin ligases. He YJ, McCall CM, Hu J, Zeng Y, Xiong Y. Genes Dev. 2006 Nov 1. 20(21):2949-54. 10.1101/gad.1483206 PubMed 17079684