|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20732||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepBluescriptII KS
- Backbone size w/o insert (bp) 2970
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)409
Entrez GeneCol1a1 (a.k.a. Col1a-1, Cola-1, Cola1, Mov-13, Mov13)
- Cloning method Restriction Enzyme
- 5′ cloning site PstI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T3 (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
There are a few mismatches between the author's sequence and Addgene's sequence. The discrepancies are in the targeting arms and will not affect function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Col1A-5'probe was a gift from Rudolf Jaenisch (Addgene plasmid # 20732 ; http://n2t.net/addgene:20732 ; RRID:Addgene_20732)
For your References section:Efficient method to generate single-copy transgenic mice by site-specific integration in embryonic stem cells. Beard C, Hochedlinger K, Plath K, Wutz A, Jaenisch R. Genesis. 2006 Jan . 44(1):23-8. 10.1002/gene.20180 PubMed 16400644