pPI896
(Plasmid
#207453)
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PurposeREMI integrated Expression for mNeonGreen-Nap1
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Depositing Lab
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Depositing OrganizationMRC Laboratory of Molecular Biology
Located in United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 207453 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 207453-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 207453-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepPI465
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Vector typeUnspecified
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameNap1
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SpeciesDictyostelium discoideum
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Tag
/ Fusion Protein
- mNeonGreen (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 207453-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 207453-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPI896 was a gift from Rob Kay (Addgene plasmid # 207453 ; http://n2t.net/addgene:207453 ; RRID:Addgene_207453) -
For your References section:
Formation and closure of macropinocytic cups in Dictyostelium. Lutton JE, Coker HLE, Paschke P, Munn CJ, King JS, Bretschneider T, Kay RR. Curr Biol. 2023 Aug 7;33(15):3083-3096.e6. doi: 10.1016/j.cub.2023.06.017. Epub 2023 Jun 27. 10.1016/j.cub.2023.06.017 PubMed 37379843
