pCB128
(Plasmid
#205137)
-
PurposeExpression of mCherry-2xFYVE in Dictyostelium
-
Depositing Lab
-
Depositing OrganizationUniversity of Sheffield
Located in the United Kingdom of Great Britain and Northern Ireland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 205137 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 205137-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 205137-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
-
Vector backbonepDM1208
- Backbone size w/o insert (bp) 7638
-
Vector typeDictyostelium expression
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert name2xFYVE (from human Hrs)
-
SpeciesSynthetic
-
Insert Size (bp)519
-
Entrez GeneHGS (a.k.a. HRS)
-
Tag
/ Fusion Protein
- mCherry (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: We have identified several potential errors in the highly non-complex and repetitive published sequence of the Dictyostelium pDM vector backbone. These are all in non-coding highly repetitive regions so likely not important for function.
DNA (Catalog # 205137-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 205137-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCB128 was a gift from Jason King (Addgene plasmid # 205137 ; http://n2t.net/addgene:205137 ; RRID:Addgene_205137) -
For your References section:
A PI(3,5)P2 reporter reveals PIKfyve activity and dynamics on macropinosomes and phagosomes. Vines JH, Maib H, Buckley CM, Gueho A, Zhu Z, Soldati T, Murray DH, King JS. J Cell Biol. 2023 Sep 4;222(9):e202209077. doi: 10.1083/jcb.202209077. Epub 2023 Jun 29. 10.1083/jcb.202209077 PubMed 37382666
