pCDH-EF1-E-NoMi-P2A-CopGFP-T2A-PuroR
(Plasmid #207805)

• Purpose: E-NoMi is a re-engineered tetraspanin scaffold tagged with bioluminescent and fluorescent reporter proteins under an EF-1α promoter.
• Depositing Lab: Koen Breyne
• Publication: Maalouf et al Mol Ther. 2023 Jul 5;31(7):2206-2219. doi: 10.1016/j.ymthe.2023.05.011. Epub 2023 May 16.

Backbone

• Vector backbone: #73022
• Backbone manufacturer: addgene
• Backbone size w/o insert (bp): 8689
• Total vector size (bp): 10933
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: E-NoMi
• Alt name: EF-1alpha driven EV reporter called Nanoluc outside mCherry inside
• Species: H. sapiens (human)
• Insert Size (bp): 2133
• Promoter: EF-1alpha
• Tags / Fusion Proteins:
  • nanoluciferase
  • 3xFlag tag
  • mCherry (C terminal on insert)
  • copGFP (C terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ATGGCGGTGGAAGGAGGAA
• 3′ sequencing primer: ACCGGTGGATCCCGGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
  • NanoLuc Luciferase Limited Use Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry