TOMM20-PuRRRE
(Plasmid
#208329)
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PurposePuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208329 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneTOMM20-N-10_mCherry
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Backbone manufacturerMichael Davidson (Addgene plasmid # 55146)
- Backbone size w/o insert (bp) 5923
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTOMM20-PuRRRE
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SpeciesH. sapiens (human)
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Tag
/ Fusion Protein
- PuRRRE (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TOMM20-PuRRRE was a gift from Kimberly Beatty (Addgene plasmid # 208329 ; http://n2t.net/addgene:208329 ; RRID:Addgene_208329) -
For your References section:
Orthogonal Versatile Interacting Peptide Tags for Imaging Cellular Proteins. Suyama A, Devlin KL, Macias-Contreras M, Doh JK, Shinde U, Beatty KE. Biochemistry. 2023 Jun 6;62(11):1735-1743. doi: 10.1021/acs.biochem.2c00712. Epub 2023 May 11. 10.1021/acs.biochem.2c00712 PubMed 37167569
