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Addgene
Orthogonal Versatile Interacting Peptide Tags for Imaging Cellular Proteins.
Suyama A, Devlin KL, Macias-Contreras M, Doh JK, Shinde U, Beatty KE
Biochemistry. 2023 Jun 6;62(11):1735-1743. doi: 10.1021/acs.biochem.2c00712. Epub 2023 May 11. PubMed Article

Plasmids from Article

ID Plasmid Purpose
208320pET28b-TinyERREBacterial expression of the His-tagged peptide TinyERRE.
208321pET28b-TREERBacterial expression of the His-tagged peptide TREER.
208322pET28b-PunyEEERBacterial expression of the His-tagged peptide PunyEEER.
208323pET28b-PuRRREBacterial expression of the His-tagged peptide PuRRRE.
208324pcDNA TfR1-TinyERREC-terminal TinyERRE-tagged transferrin receptor 1 (tag on extracellular domain).
208325pcDNA TfR1-TREERC-terminal TREER-tagged transferrin receptor 1 (tag on extracellular domain).
208326pcDNA TfR1-PunyEEERC-terminal PunyEEER-tagged transferrin receptor 1 (tag on extracellular domain).
208327pcDNA TfR1-PuRRREC-terminal PuRRRE-tagged transferrin receptor 1 (tag on extracellular domain).
208328H2B-MiniRMiniR genetically encoded tag introduced after H2B; to be used with a MiniE probe peptide.
208329TOMM20-PuRRREPuRRRE genetically-encoded tag introduced after TOMM20; to be used with PunyEEER probe peptide.
208330TREER-ActinTREER genetically-encoded tag introduced before actin; to be used with TinyERRE probe peptide.

Antibodies from Article