TREER-Actin
(Plasmid
#208330)
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PurposeTREER genetically-encoded tag introduced before actin; to be used with TinyERRE probe peptide.
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Depositing Lab
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Depositing OrganizationOregon Health & Science University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208330 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 208330-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 208330-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonemEmerald-Actin-C-18
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Backbone manufacturerMichael Davidson (Addgene plasmid # 53978)
- Backbone size w/o insert (bp) 6643
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTREER-Actin
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SpeciesH. sapiens (human)
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Tag
/ Fusion Protein
- TREER (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 208330-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 208330-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TREER-Actin was a gift from Kimberly Beatty (Addgene plasmid # 208330 ; http://n2t.net/addgene:208330 ; RRID:Addgene_208330) -
For your References section:
Orthogonal Versatile Interacting Peptide Tags for Imaging Cellular Proteins. Suyama A, Devlin KL, Macias-Contreras M, Doh JK, Shinde U, Beatty KE. Biochemistry. 2023 Jun 6;62(11):1735-1743. doi: 10.1021/acs.biochem.2c00712. Epub 2023 May 11. 10.1021/acs.biochem.2c00712 PubMed 37167569
