pDisplay-GRAB_g5-HT3.0mut
(Plasmid
#208712)
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PurposeExpresses the green 5-HT sensor GRAB_g5-HT3.0mut in mammalian cells
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Depositing Lab
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Depositing OrganizationPeking University
Located in China -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208712 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 208712-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 208712-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepDisplay
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Backbone manufacturerpDisplay
- Backbone size w/o insert (bp) 6548
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGreen fluorescent 5-HT sensor GRAB_g5-HT3.0mut
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SpeciesH. sapiens (human), M. musculus (mouse)
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Insert Size (bp)2151
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2023.05.27.542566v1 for bioRxiv preprint.
DNA (Catalog # 208712-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 208712-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDisplay-GRAB_g5-HT3.0mut was a gift from Yulong Li (Addgene plasmid # 208712 ; http://n2t.net/addgene:208712 ; RRID:Addgene_208712) -
For your References section:
Improved green and red GRAB sensors for monitoring spatiotemporal serotonin release in vivo. Deng F, Wan J, Li G, Dong H, Xia X, Wang Y, Li X, Zhuang C, Zheng Y, Liu L, Yan Y, Feng J, Zhao Y, Xie H, Li Y. Nat Methods. 2024 Apr;21(4):692-702. doi: 10.1038/s41592-024-02188-8. Epub 2024 Mar 5. 10.1038/s41592-024-02188-8 PubMed 38443508
