RC(14-3-3ε)-LgBit
(Plasmid #208926)

• Purpose: Recognition domain for in vitro RSK-activity sensor
• Depositing Lab: Attila Reményi
• Publication: Poti et al Int J Mol Sci. 2023 Oct 3;24(19):14854. doi: 10.3390/ijms241914854.

Backbone

• Vector backbone: pet-MBP
• Backbone manufacturer: Attila Reményi lab
• Backbone size w/o insert (bp): 4730
• Total vector size (bp): 5984
• Modifications to backbone: Insertion of LgBit fragment with NotI and XhoI, and then insertion of 14-3-3E with BamHI and NotI
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: 14-3-3ε
• Species: H. sapiens (human)
• Insert Size (bp): 755
• GenBank ID: NM_006761
• Entrez Gene: YWHAQ (a.k.a. 14-3-3, 1C5, HS1)
• Promoter: T7
• Tags / Fusion Proteins:
  • MBP (N terminal on backbone)
  • LgBit (C terminal on insert)
  • HISx6 (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI, XhoI (not destroyed)
• 5′ sequencing primer: malE
• 3′ sequencing primer: T7_term

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry