Phosphorylation-Assisted Luciferase Complementation Assay Designed to Monitor Kinase Activity and Kinase-Domain-Mediated Protein-Protein Binding.
Poti AL, Denes L, Papp K, Bato C, Banoczi Z, Remenyi A, Alexa A
Int J Mol Sci. 2023 Oct 3;24(19):14854. doi: 10.3390/ijms241914854. PubMed Article
Poti AL, Denes L, Papp K, Bato C, Banoczi Z, Remenyi A, Alexa A
Int J Mol Sci. 2023 Oct 3;24(19):14854. doi: 10.3390/ijms241914854. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 208926 | RC(14-3-3ε)-LgBit | Recognition domain for in vitro RSK-activity sensor |
| 208927 | VF-Sensor | in vitro RSK-Sensor for luminescent detection of RSK-activity |
| 208928 | MEF2A-Sensor | in vitro MAPK-Sensor for luminescent detection of ERK and p38-activity |
| 208929 | PDE4B-Sensor | in vitro JNK-Sensor for luminescent detection of JNK-activity |
| 208930 | FENEF-Sensor | in vitro p38-Sensor for luminescent detection of p38-activity |
| 208931 | RC(WW)-LgBit | Recognition domain for in vitro MAPK-activity sensor |
| 209184 | pet-MBP | Expression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coli |