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Phosphorylation-Assisted Luciferase Complementation Assay Designed to Monitor Kinase Activity and Kinase-Domain-Mediated Protein-Protein Binding.

Poti AL, Denes L, Papp K, Bato C, Banoczi Z, Remenyi A, Alexa A
Int J Mol Sci. 2023 Oct 3;24(19):14854. doi: 10.3390/ijms241914854. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
208926RC(14-3-3ε)-LgBitRecognition domain for in vitro RSK-activity sensor
208927VF-Sensorin vitro RSK-Sensor for luminescent detection of RSK-activity
208928MEF2A-Sensorin vitro MAPK-Sensor for luminescent detection of ERK and p38-activity
208929PDE4B-Sensorin vitro JNK-Sensor for luminescent detection of JNK-activity
208930FENEF-Sensorin vitro p38-Sensor for luminescent detection of p38-activity
208931RC(WW)-LgBitRecognition domain for in vitro MAPK-activity sensor
209184pet-MBPExpression of recombinant proteins with N-terminal MBP-tag and C-terminal Hisx6-tag in E. coli

Antibodies from Article