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(Plasmid #208931)


Item Catalog # Description Quantity Price (USD)
Plasmid 208931 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Attila Reményi lab
  • Backbone size w/o insert (bp) 4730
  • Total vector size (bp) 5339
  • Modifications to backbone
    Cloning LgBit (481 bp) with NotI and XhoI, cloning WW-domain (128 bp) with BamHI and NotI
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    WW domain of PIN1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    PIN1 (a.k.a. DOD, UBL5)
  • Promoter T7
  • Tags / Fusion Proteins
    • MBP (N terminal on backbone)
    • LgBit (C terminal on insert)
    • HISx6 (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer malE
  • 3′ sequencing primer T7_term
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RC(WW)-LgBit was a gift from Attila Reményi (Addgene plasmid # 208931 ; ; RRID:Addgene_208931)
  • For your References section:

    Phosphorylation-Assisted Luciferase Complementation Assay Designed to Monitor Kinase Activity and Kinase-Domain-Mediated Protein-Protein Binding. Poti AL, Denes L, Papp K, Bato C, Banoczi Z, Remenyi A, Alexa A. Int J Mol Sci. 2023 Oct 3;24(19):14854. doi: 10.3390/ijms241914854. 10.3390/ijms241914854 PubMed 37834301