mTB3-BC2 nanobody
(Plasmid
#210208)
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PurposeType 3 phage-display vector that contains kanamycin resistance and displays the BC2 nanobody at the N-terminus of mature protein III.
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 210208 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonemTB3
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Backbone manufacturerAddgene ID: 206144
- Backbone size w/o insert (bp) 8264
- Total vector size (bp) 8621
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Modifications to backboneInsertion of the BC2 nanobody at the N-terminus of mature protein III.
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Vector typeBacterial Expression
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Selectable markerslacZα
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameBC2 Nanobody
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SpeciesV. pacos
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Insert Size (bp)366
Resource Information
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A portion of this plasmid was derived from a plasmid made byBC2 nanobody was described by Braun et al 2016 (PMID: 26791954)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mTB3-BC2 nanobody was a gift from Brian Kay (Addgene plasmid # 210208 ; http://n2t.net/addgene:210208 ; RRID:Addgene_210208) -
For your References section:
Efficient Cloning of Inserts for Phage Display by Golden Gate Assembly. Grahn AK, Allen GL, Kay BK. Methods Mol Biol. 2023;2702:191-203. doi: 10.1007/978-1-0716-3381-6_9. 10.1007/978-1-0716-3381-6_9 PubMed 37679620
Map uploaded by the depositor.
