pTripZ RB1 WT-3XFLAG
(Plasmid #209070)

• Purpose: DOX-ON inducible expression vector that expresses RB1 WT with c-terminal 3X flag tag
• Depositing Lab: Matthew Oser
• Publication: Oser et al Cancer Discov. 2019 Feb;9(2):230-247. doi: 10.1158/2159-8290.CD-18-0389. Epub 2018 Oct 29.

Backbone

• Vector backbone: pTripZ NEO DEST
• Backbone manufacturer: modified from thermofisher
• Backbone size w/o insert (bp): 11676
• Total vector size (bp): 12736
• Modifications to backbone: LR reaction with pENTR223 RB1 WT to make pTripz RB1 WT-3X FLAG
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: human RB1 WT after LR recombination with pTripZ NEO DEST to inducibly express RB1 WT with a c-terminal 3X flag tag
• Alt name: pTripZ RB1 WT 3X FLAG
• Species: H. sapiens (human); RB1 WT
• Insert Size (bp): 2876
• GenBank ID: 5925
• Promoter: CMV
• Tag / Fusion Protein:
  • 3XFLAG (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: ATTB1
• 3′ sequencing primer: ATTB2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry