pTripZ EV
(Plasmid #209071)

• Purpose: DOX-ON inducible expression vector that has a stuffer sequence after an LR reaction and used as empty expression vector control
• Depositing Lab: Matthew Oser
• Publication: Oser et al Cancer Discov. 2019 Feb;9(2):230-247. doi: 10.1158/2159-8290.CD-18-0389. Epub 2018 Oct 29.

Backbone

• Vector backbone: pTripZ NEO DEST
• Backbone manufacturer: modified from thermofisher
• Backbone size w/o insert (bp): 11676
• Modifications to backbone: LR reaction of stuffer sequence (ENTRY vector) with pTripz NEO DEST
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: stuffer DNA sequence after LR recombination with pTripZ NEO DEST to be used as an empty vector control
• Alt name: pTripZ EV
• Species: empty vector
• Promoter: CMV
• Tag / Fusion Protein:
  • 3XFLAG (C terminal on insert)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: ATTB1
• 3′ sequencing primer: ATTB2

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry