Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||20978||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6500
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Copy numberHigh Copy
SpeciesH. sapiens (human)
Insert Size (bp)900
MutationMissing amino acid #1. Missing 3'UTR. Coding sequence only.
Entrez GeneHOXA9 (a.k.a. ABD-B, HOX1, HOX1.7, HOX1G)
/ Fusion Protein
- Flag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (unknown if destroyed)
- 3′ cloning site XhoI (unknown if destroyed)
- 5′ sequencing primer pLXSN-5
- 3′ sequencing primer MSCV-rev (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Human HOXA9-MSCV-short was a gift from Corey Largman (Addgene plasmid # 20978 ; http://n2t.net/addgene:20978 ; RRID:Addgene_20978)
For your References section:Protein kinase C-mediated phosphorylation of the leukemia-associated HOXA9 protein impairs its DNA binding ability and induces myeloid differentiation. Vijapurkar U, Fischbach N, Shen W, Brandts C, Stokoe D, Lawrence HJ, Largman C. Mol Cell Biol. 2004 May . 24(9):3827-37. 10.1128/MCB.24.9.3827-3837.2004 PubMed 15082777
Map uploaded by the depositor.