HOXB1-pSG5
(Plasmid
#20981)
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Depositing Lab
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Depositing OrganizationSan Francisco VA Health Care System
Located in United States of America -
Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 20981 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 20981-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 20981-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepSG5
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Backbone manufacturerStratagene
- Backbone size w/o insert (bp) 4076
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHOXB1
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SpeciesH. sapiens (human)
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Insert Size (bp)1000
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Entrez GeneHOXB1 (a.k.a. HCFP3, HOX2, HOX2I, Hox-2.9)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site EcoRI (not destroyed)
- 5′ sequencing primer pCAG-F
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byFulvio Mavilio
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 20981-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 20981-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HOXB1-pSG5 was a gift from Corey Largman (Addgene plasmid # 20981 ; http://n2t.net/addgene:20981 ; RRID:Addgene_20981) -
For your References section:
Erythroid-restricted expression of homeobox genes of the human HOX 2 locus. Mathews CH, Detmer K, Boncinelli E, Lawrence HJ, Largman C. Blood. 1991 Nov 1. 78(9):2248-52. PubMed 1681962
Map uploaded by the depositor.
