pHAND1-T2A-dTom-PGK-Neo
(Plasmid #209838)

• Purpose: Tomato reporter plasmid targeting the human HAND1 gene
• Depositing Lab: Matthew Birket
• Publication: Lynch et al Stem Cell Res. 2024 Mar 4;77:103342. doi: 10.1016/j.scr.2024.103342.

Backbone

• Vector backbone: pUC57
• Backbone size w/o insert (bp): 3522
• Total vector size (bp): 6935
• Vector type: Human Targeting
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HAND1-T2A-dTomato-PGK-Neo-HAND1
• Alt name: Heart and neural crest derivatives expressed 1
• Species: H. sapiens (human)
• Insert Size (bp): 4237
• GenBank ID: NM_004821 NM_004821
• Entrez Gene: HAND1 (a.k.a. Hxt, Thing1, bHLHa27, eHand)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GTAAAACGACGGCCAGT
• 3′ sequencing primer: GTCATAGCTGTTTCCTG

Resource Information

• Supplemental Documents:
  • pHAND1-T2A-dTom-PGK-Neo.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry