|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||21089||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 3000
Vector typein vitro TNT
Growth in Bacteria
SpeciesM. musculus (mouse)
Insert Size (bp)195
MutationBromo domain aa 1107-1172
Entrez GeneCrebbp (a.k.a. AW558298, CBP, CBP/p300, KAT3A, p300/CBP)
/ Fusion Protein
- Flag (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer M13_rev (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byR. Goodman and T. Kouzarides
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CBP-Bromo-SP65 was a gift from Corey Largman (Addgene plasmid # 21089 ; http://n2t.net/addgene:21089 ; RRID:Addgene_21089)
For your References section:The HOX homeodomain proteins block CBP histone acetyltransferase activity. Shen WF, Krishnan K, Lawrence HJ, Largman C. Mol Cell Biol. 2001 Nov;21(21):7509-22 10.1128/MCB.21.21.7509-7522.2001 PubMed 11585930
Map uploaded by the depositor.