pGL4.R_TRE_minCMV
(Plasmid
#211518)
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Purpose(Empty Backbone) Dual-luciferase reporter plasmid backbone for insertion of your TRE of interest driving minCMV-luc2P expression in addition to constitutive SV-40-driven Renilla luciferase expression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 211518 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGL4.33[luc2P/SRE/Hygro]
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Backbone manufacturerPromega
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Modifications to backboneHygromycin CDS was replaced with Renilla luciferase CDS. Then the minCMV promoter was ligated into the backbone following ApaI and KpnI digestion. Finally, annealed oligos containing restriction enzyme sites were ligated following KpnI digestion.
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Vector typeBacterial Expression
- Promoter SV40 and minCMV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Restriction Enzyme
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL4.R_TRE_minCMV was a gift from Justin English (Addgene plasmid # 211518 ; http://n2t.net/addgene:211518 ; RRID:Addgene_211518) -
For your References section:
Discovery and Validation of Context-Dependent Synthetic Mammalian Promoters. Zahm AM, Owens WS, Himes SR, Rondem KE, Fallon BS, Gormick AN, Bloom JS, Kosuri S, Chan H, English JG. bioRxiv. 2023 May 11:2023.05.11.539703. doi: 10.1101/2023.05.11.539703. Preprint. 10.1101/2023.05.11.539703 PubMed 37214829
Map uploaded by the depositor.
