pUPD2-HR1b-GFP-L (B3-B4)
(Plasmid
#211853)
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PurposeE3-DART degron casette for N-terminal fusion to protein of interest using Goldenbraid
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Depositing Lab
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Depositing OrganizationNorth Carolina State University
Located in the United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 211853 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 211853-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 211853-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepUPD2
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Backbone manufacturerDiego Orzaez Lab
- Backbone size w/o insert (bp) 2105
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHR1b
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SpeciesSynthetic
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Tag
/ Fusion Protein
- GFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BsmBI (destroyed during cloning)
- 3′ cloning site BsmBI (destroyed during cloning)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Rojas-Pierce Lab plasmid pMRP1178
DNA (Catalog # 211853-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 211853-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pUPD2-HR1b-GFP-L (B3-B4) was a gift from Marcela Rojas-Pierce (Addgene plasmid # 211853 ; http://n2t.net/addgene:211853 ; RRID:Addgene_211853) -
For your References section:
Rapid depletion of target proteins in plants by an inducible protein degradation system. Huang L, Rojas-Pierce M. Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072. 10.1093/plcell/koae072 PubMed 38446628
