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Addgene
Rapid depletion of target proteins in plants by an inducible protein degradation system.
Huang L, Rojas-Pierce M
Plant Cell. 2024 Mar 6:koae072. doi: 10.1093/plcell/koae072. PubMed Article

Plasmids from Article

ID Plasmid Purpose
211836pDGB3_alpha1-AtUBQ10:GFP-T35SPlant transient expression cassette for GFP (control) under UBQ10 promoter.
211837pDGB3_alpha1-AtUBQ10:HR1b-GFP-T35SPlant transient expression cassette for HR1b-GFP fusion under UBQ10 promoter.
211839pDGB3_alpha1-AtUBQ10:HR1bR181/185A-GFP-T35SPlant transient expression cassette for GFP fused to the mutant HR1b degron sequence under UBQ10 promoter.
211840pDGB3_alpha1-pAtUBQ10:HA-LRR-NEL-mcherry-TnosPlant transient expression cassette for the catalytically inactive E3-DART ligase under a UBQ10 promoter.
211841pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NEL-mcherry-TnosPlant transient expression cassette for the E3-DART ligase containing a nuclear localization signal.
211842pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL-mcherry-TnosPlant transient expression cassette for a Dexamethasone-inducible E3-DART ligase
211843pDGB3_alpha1-pAtUBQ10:HA-LRR-NELC492A-mcherry-TnosPlant transient expression cassette for the E3-DART ligase under a UBQ10 promoter.
211844pDGB3_alpha1-pAtUBQ10:3xNLS-HA-LRR-NELC492A-mcherry-TnosPlant transient expression cassette for the catalytically inactive E3-DART ligase containing a nuclear localization signal.
211845pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NELC492A-mcherry-TnosPlant transient expression cassette for a Dexamethasone-inducible E3-DART ligase, where the ligase is catalytically inactive.
211847pDGB3_alpha2-p35S:HR1bR181/185A -GFP-gRHD6-UTR-TerPlant transient expression cassette for RHD6 fused with GFP and a mutant form of the HR1b degron
211848pUPD2-GFP control (B3-B5)GFP CDS in domestication vector
211849pUPD2-N-L-mCherry2 (B5)CDS for mCherry in domestication vector
211851pUPD2-HR1b-C (B3-B4)CDS for HR1b degron sequence in domestication vector
211852pUPD2-N-L-HR1b (B4-B5)CDS for HR1b degron sequence in domestication vector
211853pUPD2-HR1b-GFP-L (B3-B4)E3-DART degron casette for N-terminal fusion to protein of interest using Goldenbraid
211855pUPD2-HR1bR181A R185A-C (B3-B4)CDS for mutant HR1b degron in domestication vector
211856pUPD2-N-L-HR1bR181A R185A (B4-B5)CDS for mutant HR1b degron in domestication vector
211857pUPD2-HR1bR181A R185A-GFP-L (B3-B4)Mutant E3-DART degron casette for N-terminal fusion to protein of interest using Goldenbraid
211859pUPD2-LRR-NEL (B4-B5)CDS for E3-DART ligase in domestication vector
211860pUPD2-HA-LRR-NEL-C (B3-B4)CDS for E3-DART ligase sequence in domestication vector
211861pUPD2-3xNLS-HA-LRR-NEL-C (B3-B4)Nuclear-localized E3-DART ligase in domestication vector.
211862pUPD2-HA-LRR-NELC492A-C (B3-B4)Mutant E3-DART ligase in domestication vector
211863pUPD2-3xNLS-HA-LRR-NELC492A-C (B3-B4)Nuclear-localized and catalytically-inactive E3-DART ligase in domestication vector.

Antibodies from Article